![]() ![]() Production of complex biopharmaceutical products rely heavily on mammalian cell lines, with most therapeutic biopharmaceuticals produced in the Chinese hamster ovary (CHO) cells ( Lalonde and Durocher, 2017). We also compare their usage against other commonly used host cell lines in each category of biotherapeutics and summarise the factors influencing the choice of host cell lines used. ![]() In this review, we discuss the use of HEK293 cells and its subtypes in the production of biotherapeutics. Among the human cell lines, HEK293 is widely utilized due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture. ![]() One important advantage of using human cell lines is the increased potential that the resulting biotherapeutics would carry more “human-like” post-translational modifications. Although most biotherapeutics are produced in animal cell lines, production in human cell lines is expanding. Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeĪnimal cell-based expression platforms enable the production of complex biomolecules such as recombinant proteins and viral vectors.Evan Tan, Cara Sze Hui Chin, Zhi Feng Sherman Lim and Say Kong Ng* ![]()
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